发布日期：2025-01-03 18:22 点击次数：72
DNA胞嘧啶的表观遗传修饰通过调节基因转录在发育中起关键作用。目前研究最多的胞嘧啶修饰是5-甲基胞嘧啶（5mC），它与基因表达的抑制有关。在脱甲基化过程中，5mC氧化为连续的中间状态，包括5-羟甲基胞嘧啶（5hmC），5-甲酰基胞嘧啶（5fC）和5-羧基胞嘧啶（5caC）。在过去的十年中，研究表明，氧化形式的5mC，尤其是5hmC，除了在活性脱甲基过程中充当“中间体”外，还可能具有多种独立的功能。很多研究表明，5hmC 表达水 平与基因表达调控、多能干细胞分化、神经元发育、以及肿瘤发生都密切相关。 而参与其中甲基化循环的一个重要酶类，就是TET家族蛋白！人TET蛋白家族包含TET1、TET2和TET3这3个成员，所有TET蛋白都包含一个保守的双链β-螺旋结构域、一个富含半胱氨酸区和C端的核心催化结构域。TET1主要在胚胎干细胞中表达，TET2主要在造血系统中表达，TET3则在 小脑、皮质和海马体等神经系统中广泛表达。结果表明，基因组5hmC 水平与 TET 羟化酶活性相关。 那么如何检测5hmc羟化酶TET的活性呢？ 作为专业的生命科学解决方案供应商，艾美捷为您推荐Nature同款方案：
Nature文章结果：DOI https://doi.org/10.1038/s41590-020-0764-8
TET酶活性分析使用P-3086; c. 使用艾美捷推荐的P-1032检测整体5hmc水平
更多客户的选择：
Qian H et. al. (March 2021). TET1 promotes RXRα expression and adipogenesis through DNA demethylation.Biochim Biophys Acta Mol Cell Biol Lipids. :158919.
Wulansari N et. al. (February 2021). LIF Maintains mESC Pluripotency by Modulating TET1 and JMJD2 Activity in a JAK2-Dependent Manner.Stem Cells.
Clark DF et. al. (January 2021). Acute high folic acid treatment in SH-SY5Y cells with and without MTHFR function leads to gene expression changes in epigenetic modifying enzymes, changes in epigenetic marks, and changes in dendritic spine densities.PLoS One. 16(1):e0245005.
Kołodziej-Wojnar P et. al. (December 2020). Alterations in the Genomic Distribution of 5hmC in In Vivo Aged Human Skin Fibroblasts.Int J Mol Sci. 22(1)
Zhou B et. al. (August 2020). The angiocrine Rspondin3 instructs interstitial macrophage transition via metabolic-epigenetic reprogramming and resolves inflammatory injury.Nat Immunol.
Rosenberg T et. al. (August 2020). Embryonic Heat Conditioning Induces TET-Dependent Cross-Tolerance to Hypothalamic Inflammation Later in Life.Front Genet. 11:767.
Gu Y et. al. (July 2020). Hydrogen sulfide attenuates renal fibrosis by inducing TET-dependent DNA demethylation on Klotho promoter.FASEB J.
Aggarwal et. al. (July 2020). Functional succinate dehydrogenase deficiency is a pathognomonic adverse feature of clear cell renal cancer
Sun X. et. al. et. al. (May 2020). AMPK deficiency induces DNA methylation and aggravates colorectal tumorigenesis in 2 AOM/DSS miceResearch Square.
Kaur G et. al. (April 2020). Regulation of DNA methylation signatures on NF-κB and STAT3 pathway genes and TET activity in cigarette smoke extract-challenged cells/COPD exacerbation model in vitro.Cell Biol Toxicol.
Briand J et. al. (April 2020). Radiotherapy-induced overexpression of exosomal miRNA-378a-3p in cancer cells limits natural killer cells cytotoxicity.
Zhou Y et. al. (March 2020). HIF1A activates the transcription of lncRNA RAET1K to modulate hypoxia-induced glycolysis in hepatocellular carcinoma cells via miR-100-5p.Cell Death Dis. 11(3):176.
Bhagat TD et. al. (October 2019). Lactate-mediated epigenetic reprogramming regulates formation of human pancreatic cancer-associated fibroblasts.8
Zhou L et. al. (October 2019). TET2-interacting long noncoding RNA promotes active DNA demethylation of the MMP-9 promoter in diabetic wound healing.Cell Death Dis. 10(11):813.
Parsanathan R et. al. (October 2019). Glutathione deficiency induces epigenetic alterations of vitamin D metabolism genes in the livers of high-fat diet-fed obese mice.Sci Rep. 9(1):14784.
Shi F et. al. (April 2019). EBV(LMP1)-induced metabolic reprogramming inhibits necroptosis through the hypermethylation of the <i>RIP3</i> promoter.9(9):2424-2438.
Cramer T et. al. (December 2018). PARP Inhibitor Affects Long-term Heat-stress Response via Changes in DNA Methylation.399:65-76.
Cramer T et. al. (October 2018). Early-life epigenetic changes along the corticotropin-releasing hormone (CRH) gene influence resilience or vulnerability to heat stress later in lifeMolecular Psychiatry.
Poupeau A et. al. (October 2018). Genes controlling the activation of natural killer lymphocytes are epigenetically remodeled in intestinal cells from germ-free mice.FASEB J. :fj201800787R.
Atlante S et. al. (July 2018). α-ketoglutarate dehydrogenase inhibition counteracts breast cancer-associated lung metastasis.Cell Death Dis. 9(7):756.
Shahid M et. al. (March 2018). Alpha-oxoglutarate inhibits the proliferation of immortalized normal bladder epithelial cells via an epigenetic switch involving ARID1A.Sci Rep. 8(1):4505.
Rea M et. al. (November 2017). Selective inhibition of CTCF binding by iAs directs TET-mediated reprogramming of 5-hydroxymethylation patterns in iAs-transformed cells.Toxicol Appl Pharmacol. 338:124-133.
Wulansari N et. al. (October 2017). Vitamin C-Induced Epigenetic Modifications in Donor NSCs Establish Midbrain Marker Expressions Critical for Cell-Based Therapy in Parkinson's Disease.Stem Cell Reports. 9(4):1192-1206.
Shenoy N et. al. (July 2017). Upregulation of TET activity with ascorbic acid induces epigenetic modulation of lymphoma cells.Blood Cancer J. 7(7):e587.
Prasad R et. al. (May 2017). Honokiol inhibits ultraviolet radiation-induced immunosuppression through inhibition of ultraviolet-induced inflammation and DNA hypermethylation in mouse skin.Sci Rep. 7(1):1657.
Röhr D et. al. (April 2017). Sodium-dependent Vitamin C transporter 2 deficiency impairs myelination and remyelination after injury: Roles of collagen and demethylation.
Kowluru RA et. al. (January 2017). Role of oxidative stress in epigenetic modification of MMP-9 promoter in the development of diabetic retinopathy.Graefes Arch Clin Exp Ophthalmol.
Ishikawa K et. al. (February 2015). Long-term pancreatic beta cell exposure to high levels of glucose but not palmitate induces DNA methylation within the insulin gene promoter and represses transcriptional activity.PLoS One. 10(2):e0115350.
Niu Y et. al. (February 2015). Oxidative stress alters global histone modification and DNA methylation.Free Radic Biol Med.
He XB et. al. (December 2014). Vitamin C Facilitates Dopamine Neuron Differentiation in Fetal Midbrain Through Tet1- aND Jmjd3-Dependent Epigenetic Control Manner.Stem Cells.
Dubois-Chevalier J et. al. (September 2014). A dynamic CTCF chromatin binding landscape promotes DNA hydroxymethylation and transcriptional induction of adipocyte differentiation.Nucleic Acids Res.
Scola G et. al. (April 2014). Lithium reduces the effects of rotenone-induced complex I dysfunction on DNA methylation and hydroxymethylation in rat cortical primary neurons.Psychopharmacology (Berl).
Nettersheim D et. al. (December 2013). Analysis of TET expression/activity and 5mC oxidation during normal and malignant germ cell development.PLoS One. 8(12):e82881.
Coulter JB et. al. (October 2013). Hydroquinone increases 5-hydroxymethylcytosine formation through ten eleven translocation 1 (TET1) 5-methylcytosine dioxygenase.J Biol Chem. 288(40):28792-800.
................................................................................................看到这儿，您心动了吗？马上联系小艾吧！或者扫描下方二维码，即可联系您的专属客服哦~ 作为一家具有高端的技术实力、先进的经营管理水平和完善的市场销售体系的生物高科技企业，总部位于武汉光谷高新技术开发区，服务面向全国。艾美捷科技是集进口试剂、实验室耗材销售、技术服务与合约开发为一体的专业化高科技公司，为用户提供专业的前沿资讯、完备的产品、整合的解决方案，及优质的物流服务。为了更好的服务客户，公司组建了一支经验丰富的研发团队-艾美捷生物技术中心，进入研发生产阶段，将更优质的产品推荐给国内生物领域的同仁们! 艾美捷科技与国内外优秀的生物试剂供应商优保持着密切的合作关系，目前已成为众多国际知名品牌的中国总代理或一级代理，主要包括：AmyJet、AAT Bioquest、Abbexa、Abnova、Agrisera、Anogen、Biosensis、Biovision、BioVendor、Caisson Labs、Cayman Chemical、Cell Biolabs、Cytoskeleton、Demeditec、Duchefa、Epigentek、Equitech-Bio、EXBio、Fitzgerald、GeneCopoeia、Hycult Biotech、ImmunoReagents、ImmunoStep、Jackson、LifeSensors、LigaTrap、Lumiprobe、Mabtech、Matreya、Norgen Biotek、Origene、ProImmune、ProSpec、ScyTek、Solis BioDyne、SouthernBiotech、StressMarq、SySy、US Biological、TRC等，可以在短时间内为用户提供专业的前沿资讯、完备的产品及物流服务。
